Semi Prep HPLC Column, HPS Silica, 5um, 120A. 10mm ID x 50mm Length. Cogent Brand. 1 EA.
Additional Info:
75001-SP50 Cogent HPS Silica™ Semi‑Prep HPLC Column is packed with high‑purity, unmodified silica, providing reliable and reproducible performance for normal phase purification of polar and hydrophilic compounds.
Because the stationary phase is not chemically modified, it enables strong surface interactions with polar analytes, making it ideal for semi‑preparative purification of lipids, fats, and other hydrophilic compounds.
This proprietary HPS Silica™ phase has been widely used by HPLC laboratories for many years as a premium-value alternative to commonly used silica columns such as Inertsil® and Luna®, delivering consistent selectivity and performance.
The 5 µm particle size is well suited for semi‑prep applications, allowing:
Higher flow rates
Lower system backpressure
Durable performance under repeated purification cycles
50 mm Length – Fast Semi‑Prep Purification
The 50 mm column length is optimized for high throughput.
Very fast purification cycles
Reduced solvent consumption
Ideal for method scouting and early-stage scale‑up
✅ Recommended for rapid purification and screening workflows
10 mm ID – Semi‑Preparative Scale
Designed for semi‑prep purification workflows
Increased sample loading vs analytical columns
Suitable for scale‑up from analytical methods
Compatible with fraction collection systems
✅ Recommended for purification and intermediate-scale separation
Flow Rate & System Requirements (10 mm ID, 5 µm)
⚠️ Requires semi‑preparative HPLC system capability
Typical flow rate range: ~4 – 12 mL/min
Low-to-moderate backpressure (5 µm particles)
Designed for higher flow purification
Compatible with preparative detectors and fraction collectors
✅ Important: A specialized HPLC system is required for columns of this size
Why Choose HPS Silica™ for Semi‑Prep
High‑Purity Unmodified Silica
Strong interaction with polar compounds
Ideal for normal phase purification
USP L3 Compatibility
Useful for compendial or validated methods
Reliable alternative to legacy silica phases
Proven Performance
Widely used in HPLC labs for many years
Consistent selectivity across batches
Performance Advantages
Low Backpressure (5 µm Particles)
Enables high flow rates for purification
Compatible with prep systems
Fast Equilibration
Reduces cycle time between runs
Strong Polar Retention
Ideal for lipids and hydrophilic compounds
Typical Applications
Semi‑preparative purification of lipids and fats
Normal phase HPLC separations
Polar compound isolation
Method scale‑up from analytical columns
Pharmaceutical and chemical purification workflows
Important Usage Notes
Designed for normal phase chromatography conditions
Use appropriate non‑polar mobile phases
Requires semi‑preparative HPLC system
Ensure proper equilibration for reproducible results
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable